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reverse transcription quantitative polymerase chain reaction pcr assay  (Bio-Rad)


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    Bio-Rad reverse transcription quantitative polymerase chain reaction pcr assay
    Reverse Transcription Quantitative Polymerase Chain Reaction Pcr Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15045 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative polymerase chain reaction pcr assay/product/Bio-Rad
    Average 99 stars, based on 15045 article reviews
    reverse transcription quantitative polymerase chain reaction pcr assay - by Bioz Stars, 2026-06
    99/100 stars

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    ( A ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with LGG for 6 hours. ( B ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI of FAM fluorescence from miRNA after coculture with LGG for 6 hours. ( C ) Confocal laser scan microscope image of uptake by LGG after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( D ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with E. coli (EcN) for 6 hours. ( E ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI analysis of FAM fluorescence from miRNA after coculture with EcN for 6 hours. ( F ) Confocal laser scan microscope image of uptake by EcN after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( G ) The putative binding sites of mdo-miR7267-3p in the monooxygenase ycnE mRNA. ( H ) Quantitative <t>polymerase</t> chain reaction <t>(qPCR)</t> analysis of ycnE expression in LGG treated with mdo-miR7267-3p formulations. ( I ) The pathway of miRNA regulation of indole-3-carboxaldehyde (I3A) production. ( J ) I3A level after cocultured with various mdo-miR7267-3p formulations. Data in (A), (B), (D), (E), (H), and (J) are expressed as means ± SD ( n = 3 biologically independent samples) and representative of two independent experiments. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons post hoc test, giving P values: * P < 0.05 and *** P < 0.001. PE, phycoerythrin; 5′UTR, 5′ untranslated region; a.u., arbitrary units.
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    ( A ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with LGG for 6 hours. ( B ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI of FAM fluorescence from miRNA after coculture with LGG for 6 hours. ( C ) Confocal laser scan microscope image of uptake by LGG after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( D ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with E. coli (EcN) for 6 hours. ( E ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI analysis of FAM fluorescence from miRNA after coculture with EcN for 6 hours. ( F ) Confocal laser scan microscope image of uptake by EcN after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( G ) The putative binding sites of mdo-miR7267-3p in the monooxygenase ycnE mRNA. ( H ) Quantitative <t>polymerase</t> chain reaction <t>(qPCR)</t> analysis of ycnE expression in LGG treated with mdo-miR7267-3p formulations. ( I ) The pathway of miRNA regulation of indole-3-carboxaldehyde (I3A) production. ( J ) I3A level after cocultured with various mdo-miR7267-3p formulations. Data in (A), (B), (D), (E), (H), and (J) are expressed as means ± SD ( n = 3 biologically independent samples) and representative of two independent experiments. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons post hoc test, giving P values: * P < 0.05 and *** P < 0.001. PE, phycoerythrin; 5′UTR, 5′ untranslated region; a.u., arbitrary units.
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    ( A ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with LGG for 6 hours. ( B ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI of FAM fluorescence from miRNA after coculture with LGG for 6 hours. ( C ) Confocal laser scan microscope image of uptake by LGG after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( D ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with E. coli (EcN) for 6 hours. ( E ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI analysis of FAM fluorescence from miRNA after coculture with EcN for 6 hours. ( F ) Confocal laser scan microscope image of uptake by EcN after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( G ) The putative binding sites of mdo-miR7267-3p in the monooxygenase ycnE mRNA. ( H ) Quantitative <t>polymerase</t> chain reaction <t>(qPCR)</t> analysis of ycnE expression in LGG treated with mdo-miR7267-3p formulations. ( I ) The pathway of miRNA regulation of indole-3-carboxaldehyde (I3A) production. ( J ) I3A level after cocultured with various mdo-miR7267-3p formulations. Data in (A), (B), (D), (E), (H), and (J) are expressed as means ± SD ( n = 3 biologically independent samples) and representative of two independent experiments. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons post hoc test, giving P values: * P < 0.05 and *** P < 0.001. PE, phycoerythrin; 5′UTR, 5′ untranslated region; a.u., arbitrary units.
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    ( A ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with LGG for 6 hours. ( B ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI of FAM fluorescence from miRNA after coculture with LGG for 6 hours. ( C ) Confocal laser scan microscope image of uptake by LGG after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( D ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with E. coli (EcN) for 6 hours. ( E ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI analysis of FAM fluorescence from miRNA after coculture with EcN for 6 hours. ( F ) Confocal laser scan microscope image of uptake by EcN after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( G ) The putative binding sites of mdo-miR7267-3p in the monooxygenase ycnE mRNA. ( H ) Quantitative polymerase chain reaction (qPCR) analysis of ycnE expression in LGG treated with mdo-miR7267-3p formulations. ( I ) The pathway of miRNA regulation of indole-3-carboxaldehyde (I3A) production. ( J ) I3A level after cocultured with various mdo-miR7267-3p formulations. Data in (A), (B), (D), (E), (H), and (J) are expressed as means ± SD ( n = 3 biologically independent samples) and representative of two independent experiments. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons post hoc test, giving P values: * P < 0.05 and *** P < 0.001. PE, phycoerythrin; 5′UTR, 5′ untranslated region; a.u., arbitrary units.

    Journal: Science Advances

    Article Title: miRNA-loaded biomimetic nanoparticles orchestrate gut microbe to ameliorate inflammatory bowel disease

    doi: 10.1126/sciadv.adw5984

    Figure Lengend Snippet: ( A ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with LGG for 6 hours. ( B ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI of FAM fluorescence from miRNA after coculture with LGG for 6 hours. ( C ) Confocal laser scan microscope image of uptake by LGG after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( D ) Flow cytometric histogram and MFI analysis of DiI fluorescence from LNPs after coculture with E. coli (EcN) for 6 hours. ( E ) Flow cytometric histogram of FAM fluorescence from miRNA and MFI analysis of FAM fluorescence from miRNA after coculture with EcN for 6 hours. ( F ) Confocal laser scan microscope image of uptake by EcN after coculture for 6 hours. Fluorescence intensity traces (orange line) from the left images were plotted at the right using ImageJ software. ( G ) The putative binding sites of mdo-miR7267-3p in the monooxygenase ycnE mRNA. ( H ) Quantitative polymerase chain reaction (qPCR) analysis of ycnE expression in LGG treated with mdo-miR7267-3p formulations. ( I ) The pathway of miRNA regulation of indole-3-carboxaldehyde (I3A) production. ( J ) I3A level after cocultured with various mdo-miR7267-3p formulations. Data in (A), (B), (D), (E), (H), and (J) are expressed as means ± SD ( n = 3 biologically independent samples) and representative of two independent experiments. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons post hoc test, giving P values: * P < 0.05 and *** P < 0.001. PE, phycoerythrin; 5′UTR, 5′ untranslated region; a.u., arbitrary units.

    Article Snippet: RNA extraction kits (catalog no. AG21022), reverse transcription reagent (catalog no. AG11706), and reverse transcription quantitative polymerase chain reaction (qPCR) kits (catalog no. AG11718) were obtained from Accurate Biotechnology (Hunan) Co. Ltd. (Changsha, China).

    Techniques: Fluorescence, Microscopy, Software, Binding Assay, Real-time Polymerase Chain Reaction, Expressing